6/15/2018

Input data

  • running phenograph on Cytotoxic T cells from non-“manual” samples
  • used following markers HLA.DR, IgD, CD19, CD3, CD4, CD8, CD45RA, CCR7, CD95, CD28, CD27

Phenograph Clusters detected

  • n = 123 samples, 1890 total phenograph clusters
  • median number of clusters per sample = 16

Characterizing Phenograph Clusters

  • For each Phenograph Cluster:
    • compute median expression (centroid) of each input marker
  • group common centroids together

Characterizing Phenograph Clusters

  • “x”-axis are individual phenograph clusters ordered by sample1,sample2,….
  • “y”-axis is the median expression of each marker within that cluster

Characterizing Phenograph Clusters

  • expression “squished” to min of -20 and max of 250
  • outlier values are assigned either min or max

Characterizing Phenograph Clusters

  • “x”-axis ordered by common phenograph clusters
  • sorta grouped by “meta” clusters

Characterizing Phenograph Clusters

  • “x”-axis are individual phenograph clusters ordered by CtlASample1,CtlASample2,….

Characterizing Phenograph Clusters

  • “x”-axis are individual phenograph clusters ordered by CtlA,CtlB,….

Characterizing Phenograph Clusters

  • “x”-axis are individual phenograph clusters ordered by CtlA,CtlB,….