6/15/2018
Input data

- running phenograph on Cytotoxic T cells from non-“manual” samples
- used following markers HLA.DR, IgD, CD19, CD3, CD4, CD8, CD45RA, CCR7, CD95, CD28, CD27
Phenograph Clusters detected
- n = 123 samples, 1890 total phenograph clusters
- median number of clusters per sample = 16
Characterizing Phenograph Clusters
- For each Phenograph Cluster:
- compute median expression (centroid) of each input marker
- group common centroids together
Characterizing Phenograph Clusters

- “x”-axis are individual phenograph clusters ordered by sample1,sample2,….
- “y”-axis is the median expression of each marker within that cluster
Characterizing Phenograph Clusters

- expression “squished” to min of -20 and max of 250
- outlier values are assigned either min or max
Characterizing Phenograph Clusters

- “x”-axis ordered by common phenograph clusters
- sorta grouped by “meta” clusters
Characterizing Phenograph Clusters

- “x”-axis are individual phenograph clusters ordered by CtlASample1,CtlASample2,….
Characterizing Phenograph Clusters

- “x”-axis are individual phenograph clusters ordered by CtlA,CtlB,….
Characterizing Phenograph Clusters

- “x”-axis are individual phenograph clusters ordered by CtlA,CtlB,….